Question

What is the mass balance equation for 1.0uM of an acidic indicator dye, HIn, with pKa=6.0,...

What is the mass balance equation for 1.0uM of an acidic indicator dye, HIn, with pKa=6.0, in aqueous solution buffered at pH 8.5. (some assumptions may be made.)

Science   Chemistry   
0 0
Add a commentTranscribed image text
Next >

Homework Answers

Answer #1

The indicator at equillibrium will have the following species:

HIn + H2O ===> In- + H3O+

Depending upon the pH of the solution, the equillibrium will be shifted either to the right or left. For example, if the pH<7 the equillibrium is shifted to the left as the H3O+ is more. On the other hand, if the pH>7 the equillibrium is shifted to the right.

Thus the mass balance equation will be,

Assumptions: In- > HIn

CHIn, In- = [HIn] + [In-] = 1.0 uM + 1.0 uM = 2.0 uM

0 0
Know the answer?
Your Answer:

Post as a guest

Your Name:

What's your source?

Earn Coins

Coins can be redeemed for fabulous gifts.

Log In

Sign Up

Not the answer you're looking for?
Ask your own homework help question
Similar Questions
What mass of solid KOH must be added to 500.0 mL of 0.45 M HC2H3O2 to...
What mass of solid KOH must be added to 500.0 mL of 0.45 M HC2H3O2 to produce a solution buffered at pH=pKa.
Aspirin is a weak acid with a pKa of 2.5. If a buffered solution of aspirin...
Aspirin is a weak acid with a pKa of 2.5. If a buffered solution of aspirin with a pH of 2.5 is diluted by a factor of 10,what is the new pH? A) 1.5 B) 2.5 C) 3.5 D) between 2.5 and 3.5 E) 7 I'm not sure what the answer is but I'm guessing it's 2.5 because according to my textbook the pH of a buffered solution doesn't change the acid/base ration, and so according to the Henderson–Hasselbalch equation...
The Henderson-Hasselbalch equation relates the pH of a buffer solution to the pKa of its conjugate...
The Henderson-Hasselbalch equation relates the pH of a buffer solution to the pKa of its conjugate acid and the ratio of the concentrations of the conjugate base and acid. The equation is important in laboratory work that makes use of buffered solutions, in industrial processes where pH needs to be controlled, and in medicine, where understanding the Henderson-Hasselbalch equation is critical for the control of blood pH. Part A.) As a technician in a large pharmaceutical research firm, you need...
The Henderson-Hasselbalch equation relates the pH of a buffer solution to the pKa of its conjugate...
The Henderson-Hasselbalch equation relates the pH of a buffer solution to the pKa of its conjugate acid and the ratio of the concentrations of the conjugate base and acid. The equation is important in laboratory work that makes use of buffered solutions, in industrial processes where pH needs to be controlled, and in medicine, where understanding the Henderson-Hasselbalch equation is critical for the control of blood pH. Part A As a technician in a large pharmaceutical research firm, you need...
Phosphoric acid (H3PO4) is a triprotic acid with the following pKa values: pKa1 = 2.15 pKa2...
Phosphoric acid (H3PO4) is a triprotic acid with the following pKa values: pKa1 = 2.15 pKa2 = 7.20 pKa3 = 12.35 a). You wish to prepare a 1.000 L of a 0.0450 M phosphate buffer at pH 6.8. Which of the following salts do you use (PICK 2) and what mass of each salt will you add to the mixture? H3PO4 (M.W.= 97.995) KH2PO4 (M.W.=136.086) Na2HPO4 (M.W.=141.959) AlPO4 (M.W.=121.953) b). A concentrated phosphate buffer known as “phosphate buffered saline” (or...
Complete and balance the following redox equation using the smallest whole number coefficients. What is the...
Complete and balance the following redox equation using the smallest whole number coefficients. What is the coefficient of I2 in the balanced equation? MnO4-(aq) + I- -->  Mn2+ + I2 (acidic solution)
Lactic acid is very good at penetrating the skin. Because of this, it is used in...
Lactic acid is very good at penetrating the skin. Because of this, it is used in a number of products for skin care, such as exfoliating creams and chemical peels, and in formulations for transdermal delivery of medications, such as topical ointments and extended release patches for delivery through the skin. Lactic acid has a pKa of 3.86 in water at 25o C. (a) The initial formulation being tested is a 1.0 wt% solution of Poly-XSeption-L® in a 0.015 M...
Part A: Balance each of the following redox reactions occurring in acidic aqueous solution. MnO4−(aq)+Al(s)→Mn2+(aq)+Al3+(aq) Part...
Part A: Balance each of the following redox reactions occurring in acidic aqueous solution. MnO4−(aq)+Al(s)→Mn2+(aq)+Al3+(aq) Part B: What mass of lead sulfate is formed in a lead-acid storage battery when 1.40 g of Pb undergoes oxidation? Part C: A meteor has a Pb−206:U−238 mass ratio of 0.840:1.00. What is the age of the meteor? (Assume that the meteor did not contain any Pb−206 at the the time of its formation.)
1. What is the pH of a 0.000682 M solution of NaOH? 2. a)What is the...
1. What is the pH of a 0.000682 M solution of NaOH? 2. a)What is the kb for F- given that the ka for HF is 6.8*10-4 b)What is the pH of a 0.292 M solution of NaF? 3. What is the pH of a 0.03 M solution of CaO? HINT: CaO is ionic and dissociates into ions. What is the oxygen ion that results? What kind of an acid or base is it? You need to write out the...
1.) You will work with 0.10 M acetic acid and 17 M acetic acid in this...
1.) You will work with 0.10 M acetic acid and 17 M acetic acid in this experiment. What is the relationship between concentration and ionization? Explain the reason for this relationship 2.) Explain hydrolysis, i.e, what types of molecules undergo hydrolysis (be specific) and show equations for reactions of acid, base, and salt hydrolysis not used as examples in the introduction to this experiment 3.) In Part C: Hydrolysis of Salts, you will calibrate the pH probe prior to testing...
ADVERTISEMENT
Need Online Homework Help?

Get Answers For Free
Most questions answered within 1 hours.

Ask a Question
ADVERTISEMENT