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1. What changes to the migration pattern would you expect if the solvent system contained more...

1. What changes to the migration pattern would you expect if the solvent system contained more water? More chloroform? TLC Analysis of Egg Lipid

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Answer #1

Egg lipid generally consists of three major components: lipids (less polar fatty compounds); cholesterol ( the most polar component due to OH groups); & palmitate (more polar than lipids)

T LC is a technique based on adsorption & desorption phenomenon. Less or non polar components are soluble in less or non polar solvents and dragged towards the solvent front. & polar components by polar solvents.

If water is more in mobile phase, the solvent becomes more polar& hence the polar components are eluted first.

If chloroform is more in amount, it dissolves non polar components in it & hence drags non polar lipids towards the solventfront.

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