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4. a. Pyruvate dehydrogenase (the complex) catalyzes which reaction? Show the substrates and products. b.Name the...

4. a. Pyruvate dehydrogenase (the complex) catalyzes which reaction? Show the substrates and products. b.Name the coenzymes required and explain the function of each one. c.List one positive and one negative allosteric modulator. d.Where in the cell does this reaction take place

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Answer #1

Pyruvate dehydrogenase complex (PDC) is a complex that converts pyruvate into acetyl-coa

B

Pyruvate dehydrogenase uses three enzymes:

E1: Pyruvate dehydrogenase which uses thiamine pyrophosphate (TPP) as its prosthetic group.
E2: Dihydrolipoyl transacetylase which uses lipoamide and coenzyme A (also known as coASH) as its prosthetic groups.
E3: Dihydrolipoyl dehydrogenase which uses flavin adenine dinucleotide (FAD) and nicotinamide adenine dinucleotide (NAD+) as its cofactors.

C.

PDC is regulated by pyruvate dehydrogenase kinase (PDK) and pyruvate dehydrogenase phosphatase (PDP), deactivating and activating it respectively.

D.

Mitochondria

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