Question

Recipes

Thorner Buffer

10% glycerol                                      

8M urea                                             

5% SDS                                               

40mM Tris pH 6.8                              

4mg/mL Bromophenol Blue              

add β-mercaptoethanol just before use

j.       How would you make 50mls of Thorner buffer? i.e. how much of each component to get a final volume of 50ml?

Answer 1