Given the principle that like (polarity) dissolves/mixes with like for organic liquids and solids, the given order in which they run in chromatography, and the molecular structures, we can evaluate the polarity of the pigments. Which of the observed spinach pigments is least polar, thus is least attracted to the stationary phase, and moves the fastest because it spends the most time in the mobile phase (flowing solvent)?
neoxanthin
carotene
chlorophyll b
pheophytin a
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