Question

How much in g of Tris HCl (HA) (157.6 MW) and Tris base (A-) (121.1 MW)...

How much in g of Tris HCl (HA) (157.6 MW) and Tris base (A-) (121.1 MW) must be added to make 1 L of 100 mM Tris buffer that is at pH7.8? pKa of Tris is 8.06

Homework Answers

Know the answer?
Your Answer:

Post as a guest

Your Name:

What's your source?

Earn Coins

Coins can be redeemed for fabulous gifts.

Not the answer you're looking for?
Ask your own homework help question
Similar Questions
you need 250ml of a 100 mM of tris buffer at pH of 8.25. the molecular...
you need 250ml of a 100 mM of tris buffer at pH of 8.25. the molecular weight of tris is 121.1 and its pKa is 8.06. A) how much tris do you need to weight out? B) you have 10 M HCL soulution, how much will you add to get the right pH?
How would you make 2 liters of a 0.5 M Tris buffer, pH 7.6? MW of...
How would you make 2 liters of a 0.5 M Tris buffer, pH 7.6? MW of Tris, C4H11NO3: 121.1
Outline how you would prepare 500 mL of the following solutions: 0.01M Tris, pH 7.5, 100...
Outline how you would prepare 500 mL of the following solutions: 0.01M Tris, pH 7.5, 100 mM NaCl 0.05 M Tris,pH 7.8, 500 mM NaCl, 20 mM CaCl2 100 mM Tris, pH 8.7, 20 mM EDTA You have the following available: Tris base (FW = 121.1 g/n, pKa = 8.21) NaCl (FW = 58.44 g/n) 100 mM CaCl2 6 M HCl 6 M NaOH 500 mM EDTA
1a) Calculate the mass of Tris Base for 100 mL of a 0.1 M Solution 1b)...
1a) Calculate the mass of Tris Base for 100 mL of a 0.1 M Solution 1b) Predict the pH after additon of 2.0 mL of 1.0 HCl (pKa of Tris 8.06) 1c) Predict the pH after addition of 6.0 mL 1.0 HCl
Buffers and pH 1.(12 pts) A stock solution of Tris -HCl buffer sits on the shelf...
Buffers and pH 1.(12 pts) A stock solution of Tris -HCl buffer sits on the shelf (0.6 M, pH 8.35). You remove 80 mL of the stock solution, add 12 mL of 0.75M HCl and dilute to 1 L. The pKa of Tris-HCl is 8.20. (a) How many millimoles of both forms of Tris -HCl are present after you added the acid? (b) What is the final buffer concentration in mM? (c)What is the final pH of your buffer? Can...
How many mmols of Tris would you need to prepare 100 mL of a 100mM Tris...
How many mmols of Tris would you need to prepare 100 mL of a 100mM Tris solution? How many grams of Tris (MW 121.1) would you need ot prepare 100 mL of a 100 mM Tris solution?
1.Show calculations for making following reagents A. 500 ml of a 1M solution of Tris (molecular...
1.Show calculations for making following reagents A. 500 ml of a 1M solution of Tris (molecular weight 121.1 g/mol)                                      1Pt B. 200 ml of 0.2M EDTA (MW 372.2 g/mol)                                                                                  1Pt C. 100 ML of TE buffer (contains 10 mM Tris and 1 mm EDTA) using A and B                  
A solution contains 32 g/L of tris(hydroxymethyl)-aminomethane, also known as TRIS base (pKb= 5.9) (Molec Wt=...
A solution contains 32 g/L of tris(hydroxymethyl)-aminomethane, also known as TRIS base (pKb= 5.9) (Molec Wt= 121.12). to 35 mL of this TRIS solution, 25 ml of 0.1N HCl solution is added and the volume is made up to 100 mL. What is the pH of the solution? It is known that one mole of TRIS base reacts with one mole of HCL to give one mole of TRIS-HCL salt
You are asked to prepare 1.2 l of a 0.05 M tris buffer at pH= 7.8....
You are asked to prepare 1.2 l of a 0.05 M tris buffer at pH= 7.8. You start with the conjugate base form of tris (121 g/mol). How many grams of tris must you weigh out? How many ml of 6 M HCl (a strong acid) must you add to reach pH=7.8? The pKa for tris is 8.1.
To prepare 100 mL of 25 mM Tris buffer at pH 8.0, calculate the amount of...
To prepare 100 mL of 25 mM Tris buffer at pH 8.0, calculate the amount of Tris base and Tris·HCl needed to make the buffer. (Hint: find out the pKa of Tris·HCl and use the Henderson-Hasselbalch Equation.) Describe how do you want to make the solution, including how much water to add, how to adjust pH, etc.
ADVERTISEMENT
Need Online Homework Help?

Get Answers For Free
Most questions answered within 1 hours.

Ask a Question
ADVERTISEMENT