Question

Oxidation NADH and FADH2 are often considered “energy currency” in cellular metabolism because they are generated...

Oxidation NADH and FADH2 are often considered “energy currency” in cellular metabolism because they are generated directly from Glycolysis and the TCA cycle. Using the following half-cell potentials, calculate the Gibbs Free Energy that can be derived from the oxidation of NADH and FADH2. Use the Faraday Constant at 96,500 J/V mol.(should get two values)

FAD + 2H+ +2 e- = FADH2 Eo = -0.219V

NAD+ + H+ + 2 e- = NADH Eo = -0.320V

Homework Answers

Answer #1

FAD + 2H+ +2 e- = FADH2 Eo = -0.219V

NAD+ + H+ + 2 e- = NADH Eo = -0.320V

NADH --------> NAD+ + H+ + 2e-   E0 = 0.326v

FAD + 2H+ + 2e- -------> FADH2 E0   = -0.219v

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                                                    E0cell = 0.11v

   n = 2

G   = -nEocell *F

             = -2*0.11*96500   = -21230J

G<0 it is spontanous reaction

           =

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