Question

For size-exclusion chromatography, how would one increase ​BOTH​ column resolution and accuracy of a molecular weight calculation of a substance?

Answer 1

These answers are fully based on the instrumental side, So to get good column resolution in size exclusion chromatography, there must be a 10% difference in molecular mass . Molecular weight can be improved based on flow rate, mobile phase and relation between concentration and sample volume.

Operate instrument at or near minimum of the van Deemter plot (HETP* vs. linear velocity),adjust peak width and area,avoid polymer-packing interactions during the mobile phase selection to get zero enthalpy change.