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Determine the subunit composition of this protein from the following information: Molecular mass of the native...

Determine the subunit composition of this protein from the following information: Molecular mass of the native protien by gel filtration shows a single band at 800 kD. Molecular mass by SDS-PAGE shows a single band at 200 kD. Molecular mass by SDS-PAGE with 2-mercaptoethanol shows 4 bands at 100kD, 60 kD, 25 kD, and 15 kD?

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Answer #1

You treat your protein with protease (e.g. trypsin), run the product of the proteolytic cleavage on the SDS PAGE, and discover that it shows 4 bands in the presence of β-mercaptoethanol (2-mercaptoethanol). Trypsin cleavage sites (Lys, Arg) are located in the polypeptide sequences of the subunits of this protein between the sites involved in disulfide bonding, so that no peptides are released after the cleavage: the cleaved parts of the sequence are still held together by disulfide bonds if you run in absence of 2-mercaptoethanol also.

The protein consists of 4 subunits with molecular weights: 700 kDa (one subunit), 60 kDa (one subunit), 25 kda (one subunit) and 15 kDa (one subunit), connected with each other by disulfide bonds.

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