Question

1. If the extraction step did not completely isolate the analyte, what effect would it have...

1. If the extraction step did not completely isolate the analyte, what effect would it have on the results from the UV‐vis and fluorimeter?

2. Propose another techniques that you could use to determine the concentration of the analyte. What are the advantages and disadvantages of the techniques used in this experiment compared to the one(s) you have proposed.

Homework Answers

Answer #1

1. If the extraction step did not completely isolate the analyte, it will highly impact the results of Uv-s and fluorimeter. Let's understand this by an example. You want to measure the UV-vis spectrum for compound A, and if the extraction step did not completely isolate A, so it may have some contamination of Impurity(I), so if you will measure the sample in UV‐vis and fluorimeter, your result will be for A + I, which will differ from pure sample A. So, it's advisable to completely isolate the analyte during extraction step.

2. Acid-Base titrations are one of the other technique to determine the concentration of the analyte.

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