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Fill in the blank. In Western blotting, the secondary antibody binds to the _________________________________________.

Fill in the blank.

In Western blotting, the secondary antibody binds to the _________________________________________.

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Answer #1

western blotting, is a widely used analytical technique in molecular biology and immunogenetics to detect specific proteins in a sample of tissue homogenate or extract.

The sample undergoes protein denaturation,and gel electrophoresis. A synthetic or animal-derived antibody (known as the primary antibody) is created that recognises and binds to a specific target protein. The electrophoresis membrane is washed in a solution containing the primary antibody, before excess antibody is washed off. A secondary antibody is added which recognises and binds to the primary antibody.

Therefore

In Western blotting, the secondary antibody binds to the primary antibody.

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