Question

What are the three steps that are used to regulate the flux through glycolysis? (BIOCHEMISTRY)

What are the three steps that are used to regulate the flux through glycolysis? (BIOCHEMISTRY)

Homework Answers

Answer #1

Preparatory phase

The first five steps are regarded as the preparatory (or investment) phase, since they consume energy to convert the glucose into two three-carbon sugar phosphates

Pay-off phase

The second half of glycolysis is known as the pay-off phase, characterised by a net gain of the energy-rich molecules ATP and NADH.[2] Since glucose leads to two triose sugars in the preparatory phase, each reaction in the pay-off phase occurs twice per glucose molecule. This yields 2 NADH molecules and 4 ATP molecules, leading to a net gain of 2 NADH molecules and 2 ATP molecules from the glycolytic pathway per glucose.

Regulation

Glycolysis is regulated by slowing down or speeding up certain steps in the pathway by inhibiting or activating the enzymes that are involved. The steps that are regulated may be determined by calculating the change in free energy, ΔG, for each step

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