Question

1. please answer TRUE or FALSE

1) In size exclusion chromatography, the smallest proteins are eluted last.

2) Single-stranded RNA molecules can have extensive regions of intramolecular base pairing leading to defined secondary and tertiary structure.

2. Applications of mass spectrometry include:

detection of post-translational modifications on proteins.

determination of the mass of a protein.

determining the primary structure of proteins.

A and B.

A, B, and C.

3. Please answer the blank

The nucleotide sequence of a polynucleotide chain is called the ________ structure.

Answer 1

1.This statement is FALSE

Because in Size Exclusion Chromatography , Especially we use matterial with the pores are used to fills the column. When the molecules flow in the column , the smaller molecules retarded due its small size and can be easily inserted into the pores ,Hence the molecule with lagrer size are filled first then small molecules will be eluted.

2. This statement is TRUE

Becuase RNA are mainly found in Primary, secondary and tertiary forms

The Single strand RNA leads to form Secondary and teriary RNA easily due to its availbale region at one end.