Give the amino acid sequence of an octapeptide that contains the amino acids Phe, Lys, Thr, Trp, His (2 equiv), Gly, Ser, and forms the following fragments when partially hydrolyzed with HCl: Thr―Phe―Lys―Gly, Trp―His―Ser, and Ser―His―Thr―Phe.
On careful analysis of the fragments, we can see an order in the arrangement,
Please see fragment one and 3
We can see Thr is coming after Phe in both the case., then after 3 we can add the residuals of the one
Ser-His-Thr-Phe-Lys-Gly
these amino acids are arranged in the above sequence in the octapeptide.
By seeing the amino acid fragmnets 2 and 3 Trp and His are added to Ser (presence of second His is clear--One his is bonded to ser and Thr and other is Ser and Thp)
Thus
octapeptide is
Trp-His-Ser-His-Thr-Phe-Lys-Gly
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