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Which of the following is the definition of gradient elution? In a gradient elution, the solvent...

Which of the following is the definition of gradient elution?

In a gradient elution, the solvent composition is held constant throughout the elution.

In a gradient elution, the applied two or more solvents should have similar polarities.

In a gradient elution, two or more solvents are employed and the composition of the eluent is changed continuously or in steps as the separation proceeds.

In a gradient elution, only two solvents are employed and the composition of one is changed while the composition of the other is held constant.
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Answer #1

The definition of the Gradient elution suits the last option

In a gradient elution, only two solvents are employed and the composition of one is changed while the composition of the other is held constant.

In gradient elution is the one of chromatographic separation in which the mobile phase composition is changed during the separation process.

For example, if a gradient elution starting at 10% methanol and ending at 90% methanol after 20 minutes. Here change in the composition of methanol happened, while the other solvent composition kept constant.

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