Question
The initial rate for an enzyme-catalyzed reaction has been determined at a number of substrate concentrations....
The initial rate for an enzyme-catalyzed reaction has been determined at a number of substrate concentrations. Data are as follows:
[S](μmol/L) v[(μmol/L)min−1]
5 22
10 39
20 65
50 102
100 120
200 135.
a) Using Excel or any graphing software, create the Lineweaver-Burk plot and estimate Vmax.
b) Using the same Lineweaver-Burk plot that you created, estimate KM.
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