Question

You wish to perform an ion exchange purification of a peptide having the sequence: GLEKSLVRLGDVQPSLGKESRAKKFQRQ. You have two different ion exchange resins available: DEAE – diethylaminoethyl coated resin; and CM – carboxymethyl coated resin. Which would you choose to separate out the peptide described above? Justify your choice using proper chemistry terminology.

Answer 1

Which would you choose to separate out the peptide described above?

i will choose CM (- charge)

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CMC

1. Insoluble microgranular in nature.
2. It is used as a cation-exchange resin ……………….in ion-exchange chromatography for purification of proteins.
3. the level of derivatization is much lower, so the solubility properties of microgranular cellulose are retained.
4. By addition of negatively charged carboxylate groups to bind to positively charged proteins.