Question

FOR ATP ACCOUNTING QUESTIONS, ASSUME THAT "cytosolic NADH" (NADH produced in glycolysis) ENTERS MITOCHONDRIA WITHOUT USE OF ENERGY -- and therefore without lowering the eventual ATP yield. That is, assume that the malate-aspartate shuttle is used to move cytosolic NADH into mitochondria.

Phosphorolysis produces glucose-1-phosphate. Calculate the yield of ATP equivalents from complete aerobic catabolism of glucose-1-phosphate.

A. 3

B. 8

C. 30

D. 31

E. 32

F. 33

Answer 1

glucose-1-phosphate   -----> glucose-6-phosphate

glucose-6-phosphate    ----->   fructose-6-phosphate

fructose-6-phosphate + ATP -----> fructose- 1,6-bisphosphate

fructose- 1,6-bisphosphate -----> glyceraldehyde-3-phosphate (GAP) + dihydroxyacetone phosphate (DHAP)

glyceraldehyde-3-phosphate (GAP) + dihydroxyacetone phosphate (DHAP)+ 4 ADP – 4Pi + 2NAD+ ----->----->-----> 2 Pyruvate + 4 ATP + 2NADH

Until there 8 ATP molecules (4 ATP - 1ATP + 2*2.5 ATP = 8)

Pyruvate + NAD+ --à ACetilCoA + NADH

ACetilCoA + 3 NAD+ + FAD + GDP       ----> Oxoloacetate + 3 NADH + FADH    + GTP

Until here 10 ATP (from 4 NADH) + 1.5 ATP (From 1 FADH) + 0.5 ATP (from GTP) per each pyruvate

So 24 ATP for the citric acid

I get 33 ATP in total

Answer E (32 ATP)