Question

A) Woud N-1,5-dimethylhexylformamide be an effective alternative for the treatment of methanol and ethylene glycol poisoning, assuming that it is non-toxic itself (and as an alternative to getting the patient drunk)? Would N-1,5-dimethylhexylformamide be effective even if the concentrations of methanol or ethylene glycol were very high? (Hint: Compare the values of KI and KI' whichever is appropriate and Km). B) The compound 4-methyl pyrazol eis curently being used as a treatment for methanol poisoning. How owuld the effectiveness of 4-methyl pyrazole compare with the effectiveness of a formamide treatment?

Answer 1

a)

As it is non-toxic itself., N-1,5-dimethylhexylformamide would be an effective alternative for the treatment of methanol and ethylene glycol poisoning,

The action of N-1,5-dimethylhexylformamide is similar to alcohol dehydrogenase present in our liver.

It acts as uncompetitive inhibitor for treatment of methanol and ethylene glycol poisoning.

K_M of ADH1 without inhibitor = 0.3069

K_M of ADH1 with inhibitor = 0.1157 ,

so it is effective inhibitor to treat the alcohol poisoning.

b)

If we compare the effectiveness of 4-methyl pyrzole , it becomes ineffective high substrate concentration while formamides being having action of binding with E-NADH complex in place of free enzyme or enzyme substrate complex they act as non competitive. T

hey are analog to aldehyde.