Question

1.

a. Which plot, Michaelis-Menten or Lineweaver-Burke, yields more accurate kinetic parameters (such as KM and Vmax)? Explain your reasoning and reconcile any discrepancies between the two types of graphical analyses.

b. How do the results for KM and Vmax compare between the MM and LB plots?

Answer 1

solution:

1.

a.

-Lineweaver-Burk plot is the graphical representation of the enzyme kinetics. It is also known as double reciprocal plot.

This plot provides a good graphical representation of the analysis of Michaelis-Menton equation.

In this plot the Y-intercept is equivalent to the inverse of Vmax and the X-intercept of the graph represents −1/Km. It also gives a quick, visual impression of the different forms of enzyme inhibition.

--Michaelis-Menten plot is not accurate when compared to other. It is less used than the Lineweaver-Burk plot.

In this plot the Vmax and Km are directly obatined.

Lineweaver-Burk plot is more accurate.

b.