What is the principle behind the binding of horseradish peroxidase to concanavalin-A? Why did the sugar elute the enzyme?
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The basic principle behind the binding of horseraddish to concanavaline A, is the structure stability goes up when bonding is achieved. Binding is very specific and secondary interactions in the binded structure improves the overall stability of the structure resulting from it.
The sugar elute the enzyme, because not all the sugars bind to the enzyme. The enzyme binds to few sugars and the remaining free enzyme can be easily eluted out of the system. (a site relevant to this and references therein http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-07932003000200001
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