Protocol to make 1% SDS
SDS means standard solution.
1%SDS means 1g of solute disolves in 100 ml of solvent.
Protocol :
1. Before going to weigh the sample, we should ensure whether the weighing machine is calibrated or not.
2. If it is calibrated, then go for weighing of the sample.
3. Ensure that whether the weighing machine is in air free zone or not.
4. Weigh 1g of the sample and transfer into a clean and dried standard flask.
5.Dissolve the contents by adding 50 ml of the solvent.
6.Stirr and Shake well until the contents completely dissolves in the solvent.
7.Now make up the solution up to the mark of the Standard flask.
8.Now the standard flask contains 1%SDS.
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