When does Beer’s law fail? How can this be avoided when performing a colorimetric analysis experiment?
Beer's law falls in the given cases.
1. For high concentrated solution .
2. If the solution is turbid.
3. Presence of impurities in the sample.
4. Monochromatic light is not used.
5. Presence of stray light.
6. If chemical reaction occurs.
7. If association or dissociation of molecules occurs.
Avoidation of deviation :
1. The solution must be dilute. ( concentration < 10 mM)
2. The solution must be clear.
3. Make sure there is no stray light.
4. There should be no impurities in the sample.
5. Make sure monochromatic light has been used.
6. No chemical reaction occurs.
7. Cuvett should be placed rightly.
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