Question

In a Sep Pak c18 cartridge syringe column chromatography , Once the grape drink passes through the column the liquid coming out is clear why?

Answer 1

Solution:-

The Sep-Pak C18 cartridge syringe column chromatography used to separate many different polarity mixtures.

The Sep-Pak C18 cartridge syringe column is very non polar so once grape drink which include include sugar, citric acid, dye, and ascorbic acid passes through the very non-polar Sep-Pak C18 column, the polar molecules such as citric acid more likely bind to the polar solvent i.e water and the non-polar molecules, such as the dyes, which are not binding to the polar solvent and therefore stay in the non-polar column.Hence clear liquid coming out through the column.