Question

Primary antibody used in western blots are generated in lab animals whereas for the ELISA primary antibodies come from ____

Answer 1

Primary antibody used in Western blotting are generated in lab animals. Whereas for the ELISA also primary antibodies are comes froms lab animals because primary antibodies are A primary antibody directly binds to specific antigens, with high specificity and affinity, for the purposes of purifying or detecting and measuring the antigens. Primary antibodies can either be developed as monoclonal antibodies, which bind to one specific epitope on the antigen, or polyclonal antibodies, which bind to multiple different epitopes on the antigen, using animals as the hosts, typically: rats, rabbits, mice, goats, and other species.