Question

explain how ssRNAs can be used to modify gene expression ?

explain how ssRNAs can be used to modify gene expression ?

Homework Answers

Answer #1

The ssRNA probe is made complementary to a small region of the mRNA of the gene which expression we want to modify.

The another way is using CRISPR/CAS 9 system, the small region of the guide RNA is made complementary to the one strand of the gene. guide RNA binds on the complementary site, recruits CAS9 protein and make double stranded break in between the gene. This methid can be used in knock down of gene, modifications of the gene.

The another way is using RNAi method. Short siRNA is made that is complementary to the small region of the target mRNA. After bindinf to the target, it recruits RISC protein which then cut the mRNA from that site. From this method you can do gene silencing.

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