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Explain the process of PCR

Explain the process of PCR

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Answer #1

PCR = Polymerase chain reaction
First developed by Kary Mullis
It is an in vitro DNA amplification method

Components:
1. Template DNA
2. Primers
3. Buffer
4. dNTPs
5. Taq polymerase

Steps:
1. Denaturation (94'C)
2. Annealing (50-60'C)
3. The extension (72'C)
4. Repeat

Primers bind specifically to the region of interest in the denatured DNA. Taq polymerase extends the primer in 5'-3' direction using dNTPs as substrates.

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