Question

35a. You have added a drug that reduces the activity of the enzyme topoisomerase (gyrase). What will happen to the rate of activity of the helicase at the replication fork? Explain your reasoning.

35b. You have added a drug that reduces DNA Ligase activity. What will happen to the rate at which Okazaki fragments are joined on the lagging strand? Explain your reasoning.

35c. You have added a drug that reduces DNA Ligase activity. What will happen to the rate at which Okazaki fragments are synthesized on the lagging strand? Explain your reasoning.

Answer 1

35a)Topoisomerase is an important enzyme handling the tension of the DNA strands while occuring a replication fork for a successfull replication of Daughter DNA.When we add a drug to reduce it then the angle between the fork will decreae and replication will be not carried to its full course.

35b)DNA Ligase helps to glue the fragements,but when we add a drug to reduce its activity then the ligation will not be processed and there will be failure in production in a successfull daughter DNA

35c)There will be no change in rate of production of okazakhi fragements because DNA ligase activity is shown for ligation and not for the synthesis of DNA fragements,if we add a drug to it.