Question

In using the spectrometer, why were blank tubes used first? Select one: a. Blank tubes measure...

In using the spectrometer, why were blank tubes used first?

Select one:

a. Blank tubes measure the absorbance of the air, which is directly related to the absorbance of chlorophyll.

b. Blank tubes were used as controls in the experiment.

c. Blank tubes help the machine warm up because they do not have algae, which generates heat.

d. Blanks are used to calibrate the machine.

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Answer #1

Question: In using the spectrometer, why were blank tubes used first?

Answer: d. Blanks are used to calibrate the machine.

Explanation:To obtain thenabsorbance value of the sample, we need to subtract the blank's value out of the total absorbance.So, the machine need to be calibrated against the blank.

For example, If there is a solution (salt+water) and we want to calculate the absorbance of salt.We need to calibrate the machine with a blank(i.e. cuvette with only water), so that while calculating the absorbance of only salt out of the total absorbance, we will subtract the absorbance value we got after calibrating the machine with a blank.

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