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Which of the following statements is true? Question 1 options: a) DNA renaturation takes longer than...

Which of the following statements is true?

Question 1 options:

a) DNA renaturation takes longer than
denaturation.

a) DNA renaturation and denaturation take about the same amount of time.

a) DNA denaturation is irreversible.

a) DNA renaturation requires an input of energy.
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Answer #1

Option (a) is correct : DNA renaturation takes longer than denaturation.

DNA denaturation or melting is defined as separation of two strands of DNA to form single strands. DNA renaturation is a spontaneous reversible process, and is performed under controlled conditions of pH and ionic strength. Also, the temperature has to be gradually decreased, because of which renaturation is a slow process. The rate of renaturation depends on the type of sequence present in the DNA strand, unique sequences take more time to reanneal than do repetitive sequences.

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