Question

A mutation prevents expression of the CAP protein. Will the Lac operon be expressed when lactose...

A mutation prevents expression of the CAP protein. Will the Lac operon be expressed when lactose levels are high and glucose levels are low? Explain your reasoning.

Homework Answers

Answer #1

If the CAP protein ( catabolite activator protein) is not expressed the trancription of lac operon takes places at very low rate when lactose levels are high and glucose levels are low. It is because CAP protein is a transcriptional activator which enhances the RNA polymerase activity by binding to the promoter region of lac operon and strengthens the expression of lac operon when lactose levels are high and glucose levels are low. In the absence of CAP, the RNA polymerase trancriptional activity is not enhanced hence the transcriptional rate will be way lower.

Know the answer?
Your Answer:

Post as a guest

Your Name:

What's your source?

Earn Coins

Coins can be redeemed for fabulous gifts.

Not the answer you're looking for?
Ask your own homework help question
Similar Questions
754. A mutation in the Lac repressor protein prevents it from binding to lactose. Will the...
754. A mutation in the Lac repressor protein prevents it from binding to lactose. Will the Lac operon be expressed when lactose levels are high and glucose levels are low? Explain your reasoning.
1. The lac operon responds to lactose and glucose. If wild-type E. coli cells are grown...
1. The lac operon responds to lactose and glucose. If wild-type E. coli cells are grown in media that contains both lactose and glucose, they will consume the glucose first because: (select all that apply) A.) the lac repressor remains bound to lacO, preventing the lac operon from being expressed at maximal levels B.) the permease (lacY) is not expressed in the presence of glucose, preventing lactose from entering the cell and being converted to allolactose, the inducer C.) the...
The lac operon consists of several genes that encode enzymes required for metabolizing lactose. The expression...
The lac operon consists of several genes that encode enzymes required for metabolizing lactose. The expression of the lac operon is regulated by the presence of lactose and glucose. 1) describe the regulation operon when the level of glucose is high and the level of lactose is low
The lac operon in E. coli regulates the co-expression of three genes needed for the degradation...
The lac operon in E. coli regulates the co-expression of three genes needed for the degradation of lactose (but absence of glucose) in the growth medium. These genes are not expressed when the bacteria has no lactose to feed on. How does the cell prevent expressing the lac operon in the absence of lactose? How does the presence of lactose activate the transcription of these three genes? (You do not need to explain the glucose level regulation.)
Which of the following accurately describes the status of lac operon regulation when E. coli cells...
Which of the following accurately describes the status of lac operon regulation when E. coli cells shift from using glucose to lactose as their carbon source? A. The lac repressor is active and the CAP protein is active, so the operon is transcribed B. The lac repressor is active and the CAP protein is inactive, so the operon is transcribed C. The lac repressor is inactive and the CAP protein is active, so the operon is transcribed D. The lac...
If E. coli were subjected to high levels of lactose and low levels of glucose, which...
If E. coli were subjected to high levels of lactose and low levels of glucose, which of the following conditions would apply? Y = yes, N = no A) lac repressor would be bound to the operator of the lac operon promoter B) CAP protein would be bound to the CAP binding site adjacent to the lac operon promoter C) cAMP levels would be low D) the lac operon would be actively expressing the operon into separate mRNA molecules needed...
You isolated a mutant strain of E. coli in which the lac operon is constitutively expressed....
You isolated a mutant strain of E. coli in which the lac operon is constitutively expressed. To understand the nature of this defect, you created a partial diploid by introducing a plasmid containing a normal lacI gene (i.e. lacI+) and Z-. You then compared the mutant strain and the partial diploid with regard to their β-galactosidase activities in the presence and absence of lactose. You obtain the following results:                Lactose                        β-galactosidase expression Mutant                                     No                               High (constitutive expression) Mutant                                     Yes                             ...
Which of the following statements about regulation of the lac operon is true? Select all that...
Which of the following statements about regulation of the lac operon is true? Select all that apply. Group of answer choices When glucose is present but lactose is absent, gene expression is activated at the lac operon. The presence of glucose in the growth medium does not affect the level of gene expression activated by the presence of lactose. The lac repressor can dissociate from the operator in the presence of glucose. Gene expression is high when the lac repressor...
Compare the regulation of the lac operon and the effect on the production of ß-galactosidae when...
Compare the regulation of the lac operon and the effect on the production of ß-galactosidae when E. coli is grown under the following conditions: a) In the presence of high levels of lactose and absence of glucose b) In the presence of high levels of lactose and high levels of glucose
Discuss the organization and function of the lac operon. Include all of the important factors and...
Discuss the organization and function of the lac operon. Include all of the important factors and how they interact to regulate the expression of these genes. Discuss the following three scenarios: (i) low lactose, high glucose; (ii) high lactose, high glucose, (iii) high lactose, low glucose.