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This question refers to the lac operon. Select the correct phenotype for the genotype: I--P+OcZ--/I+P+O+Z+ [Terminology:...

This question refers to the lac operon. Select the correct phenotype for the genotype: I--P+OcZ--/I+P+O+Z+

[Terminology: β Galactosidase activity = β Gal activity]

Select one:

no β Gal activity, with no lactose (--) , β Gal activity with lactose (+)

no β Gal activity, with no lactose (--) , no β Gal activity with lactose (--)

β Gal activity, with no lactose (+) , β Gal activity with lactose (+)

β Gal activity, with no lactose (+) , no β Gal activity with lactose (+)

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Homework Answers

Answer #1

lac operon is inhibited by lac repressor by binding to the lac operator and lactose inhibits lac repressor so lac operon is expressed in the presence of lactose.

I-P+OcZ-/I+P+O+Z+

the first operon has constitutive active lac operon but this lac operon cannot express beta-galactosidase because lac Z gene is mutated, in the second operon the regulatory components, lac I, lac P, lac O are functional if lactose is present, it inhibits the lac repressor encoded by lac I gene so lac repressor cannot bind to the lac O, lac operator, so this lac operon is expressed if lactose is present, this operon has functional beta-galactosidase so beta-Gal activity is seen in the presence of lactose.

a) no β Gal activity, with no lactose (--) , β Gal activity with lactose (+)

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