Question

I want to study the activity of membrane protein; a) Which type of detergent should i...

I want to study the activity of membrane protein;
a) Which type of detergent should i use (ionic or non ionic) shall i used for isolation?
b)I want to determine the size of a membrane protein which type of detergent shall i used for the isolation?
(Can you explain me with details for me to understand it better)

Homework Answers

Answer #1

Ans-1- ionic detergent like SDS are very efficient in membrane protein extraction but they denatured the protein due which protein's native structure get disturbed and protein becomes non-functional and no activity is observed. So if you want to study membrane protein activity you can't use ionic detergent. Always use non-ionic detergent it keeps proteins in its native state and protein remains active.

Ans-2- if you want to determine the size of protein its means if protein is inactive it doesn't matter. So you can use ionic detergent which denature the proteins but does not affect the size. Ionic detergent are very efficient in membrane protein extraction by solubilizing the membrane.

Know the answer?
Your Answer:

Post as a guest

Your Name:

What's your source?

Earn Coins

Coins can be redeemed for fabulous gifts.

Not the answer you're looking for?
Ask your own homework help question
Similar Questions
You want to isolate a functional integral membrane protein from the cell to study its channel...
You want to isolate a functional integral membrane protein from the cell to study its channel activity. What method would you use to isolate the integral membrane potential? By adding SDS By using Beta-mercaptoethanol By boiling By using a mild-detergent By adding high concentration of salt
I want scenario for role play about type of conflict which is smoothing.. the scenario should...
I want scenario for role play about type of conflict which is smoothing.. the scenario should be between nurses
I am a cancer researcher. I have an antibody that recognizes the extracellular portion of Notch...
I am a cancer researcher. I have an antibody that recognizes the extracellular portion of Notch and I have conjugated it to a light producing chromophore. I know activated Notch is implicated in many types of tumors. I want to detect for a given tumor whether Notch activity has changed in comparison to wild type cells of the same type. Based upon what you know, which of the following statements could be true? (Hint: Use the information from the other...
1. How can a fusion protein be used to study the localization of a protein in...
1. How can a fusion protein be used to study the localization of a protein in a living cell by fluorescence microscopy? 2. An ionic bond is about three times stronger than a hydrogen bond. Why then is water able to separate sodium and chloride ions to dissolve salt? 3. How can you identify potential hydrogen bonding donors and acceptors in a given structure, such as an amino acid? 4. NADH/NAD+ and NADPH/NADP+ are only different in a single phosphate...
When considering a change, the nurse understands the importance of selecting the appropriate goal. Which should...
When considering a change, the nurse understands the importance of selecting the appropriate goal. Which should the nurse ask? “How much time will it take to implement this change?” “What makes me think this will solve the problem?” “How will I determine if the goal is met?” “Is this something I can really do?” 2. A nurse practices the tenets of cultural humility by: Regularly performing self-reflection to better understand personal values and biases. Taking an in-depth study of comparative...
I need assistance with this question: A psychology professor is interested in whether implementing weekly quizzes...
I need assistance with this question: A psychology professor is interested in whether implementing weekly quizzes improves student learning. She decides to use the weekly quizzes in one section of her introductory psychology class and not use them in another section of the same course. Which type of quasi-experimental design do you recommend for this study? I understand that it should be the non-equivalent control group design, because the groups are not randomly assigned, and there is a treatment group...
Activity 5             For this week’s activity, I want you to read a proposal / idea...
Activity 5             For this week’s activity, I want you to read a proposal / idea for a study and write about the “fatal flaw” in their design. This is a fun way to investigate the potential confounding or extraneous variables to the hypothesis that is being tested in an experiment. This is also a good way to show in a concrete way the considerations that researchers make on a regular basis. Please read the following prompt and answer the...
The level of monoamine oxidase (MOA) activity (nmol/hr/mg protein) was recorded for fourteen non-responsive depressed patients...
The level of monoamine oxidase (MOA) activity (nmol/hr/mg protein) was recorded for fourteen non-responsive depressed patients who had been treated with phenylzine. MOA activity is assumed to follow a normal distribution. The data are stored in a single column of Table C. You are asked to calculate a point estimate and an interval estimate of the mean MOA activity of this type of patient. Nothing is known about the variability of MOA activity.  Copy the MOA activity data from...
Review the   PILOT DOSE-RESPONSE STUDY OF MEMOROL Consent Form (ICF) Check  to determine whether the required ICH-GCP...
Review the   PILOT DOSE-RESPONSE STUDY OF MEMOROL Consent Form (ICF) Check  to determine whether the required ICH-GCP elements are present by checking Yes or No in the Informed Consent Form Checklist’. If you review an element and are not convinced that the ICF adequately covers the details– indicate this on the review form by making a note in the “comments section. Introduction: I have been asked to participate in a 1 month research study which will evaluate the effectiveness and safety...
Note: Do not use classes or any variables of type string to complete this assignment Write...
Note: Do not use classes or any variables of type string to complete this assignment Write a program that reads in a sequence of characters entered by the user and terminated by a period ('.'). Your program should allow the user to enter multiple lines of input by pressing the enter key at the end of each line. The program should print out a frequency table, sorted in decreasing order by number of occurences, listing each letter that ocurred along...