Question

Why does growth of ETPUM in Medium #1 require oxygen? Think about this in terms of how ETPUM can generate a net gain in ATP by processing polyurethane. Remember that the degradation of polyurethane by polyurethanase does not expend ATP. In order to answer this question, address each of the following questions in your answer: a. Is there a net gain or loss of ATP during the transport of the citrate? b. Consider the ATPs that can be generated via substrate-level phosphorylation. Will glycolysis be useful for generating any ATPs during growth on polyurethane? How many ATPs can be generated via the citric acid cycle? Is this enough to support growth (is there a net positive in the ATP total)? c. Now consider how else ETPUM can generate ATPs (if not by substrate- level phosphorylation). Can this process generate a net positive in the ATP tally? d. Now explain the importance of oxygen as relates to the ATP tally.

ELVIS meltdown Case Study

Answer 1

a.There results in a net loss of 2 ATP molecules.

b.Yes, glycolysis would be useful for generating ATP as the process results in a net gain of ATPs. Per cycle of glycolysis ,2 ATP molecules would be generated.

2 ATPs via citric acid cycle would be generated.

Yes, it would be would be enough to support growth.

If not by substrate- level phosphorylation, ATPs can be generated by ETC.No, this process can generate a net positive in the ATP tally,

The importance of oxygen as relates to the ATP tally would be that oxygen is more efficient for ATP production than ETC.