Question

The Weinberg lab has identified a new oncogene using the NIH 3T3 foci assay.  They are interested in whether this oncogene functions in the same pathway as the Ras oncogene.  To establish if the oncogene works in the same pathway as Ras, they should (chose the best answer)

Select one:

a. express the gene in a yeast artificial chromosome

b. make a cDNA copy of the gene and express it in E. coli

c. use CRISPR to delete the gene

d. combine it with an activated Ras oncogene and see if the combination could induce foci formation in normal human fibroblasts

e. sequence the oncogene to see if it encodes a GTPase

Current genome sequencing efforts typically involve

Select one:

a. So many genomes have been sequenced that there are really no more genome sequencing efforts

b. a few capillary sequencers running automated Sanger sequencing reactions

c. next-gen sequencing machines such as the Ion Torrent and the Illumina Hiseq

d. massive numbers of acryamide gels running radioactive Sanger sequencing reactions

e. huge numbers of acrylamide gels running Maxam-Gilbert sequencing reactions

Answer 1

The Weinberg lab has identified a new oncogene using the NIH 3T3 foci assay.  They are interested in whether this oncogene functions in the same pathway as the Ras oncogene.  To establish if the oncogene works in the same pathway as Ras, they should (chose the best answer)

e. sequence the oncogene to see if it encodes a GTPase.

Current genome sequencing efforts typically involve:

c. next-gen sequencing machines such as the Ion Torrent and the Illumina Hiseq