Question

- Explain briefly the properties of Primary antibody as compare to a secondary antibody in an immunoassay technique.

Answer 1

An immunoassay tests uses antibody and antigen complexes for generating measurable result .

Antibodies are used for research . There are two types of antibodies that is primary antibodies and secondary antibodies .primary antibodies bind to the protein or antigen . secondary antibodies are those binding to primary antibodies .

In immunoassay antibodies are labelling.

Whether to label the primary antibody or the secondary antibody depends on your experiment . both of them has its advantages and disadvantages .