Question

A coworker supplies you with a PCR product of a gene that encodes a protein your company wants to overproduce. You are told the DNA has ONLY the coding sequence of the gene. You must be sure the plasmid you choose to ligate this fragment into has an appropriately placed Question 5 options:

A) Antibiotic resistance gene

B) Promoter

C) Ribosome binding site

D) B and C

E) All of the above

Answer 1

The correct answer is the option E.

All of the above are required to be present in a plasmid which is to be used in a biotechnology experiment.

The DNA segment or the gene which was obtained in the PCR has the desirable character. So it will be unserted into a plasmid. The plasmid after forming the recombinant is incorporated into any host cell which divides rapidly.

Fir this the plasmid should have which promoter region, where the RNA polymerase binds and transcription can start. Antibiotic resistance gene is useful in testing whether the recombinant formation took place successfully or not. Ribosome binding site is required for the translation process.

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