which of these is a component used in our PCR reaction?
A. wash buffer
B. DNA polymerase
C. elution Buffer
D. all options are correct
Ans :- all options are correct
For PCR reaction the components are used that are given below -
PCR PRIMERS
TEMPLATE DNA
PCR BUFFER
TAQ POLYMERASE
NUCLEOTIDES
Temperature is necessary to separate two complementary strands of DNA and Dna polymerase is used as a component in PCR reaction that can tolerate high temperature upto 95 degree C.Wash buffer is a tris buffer solution Ph is near to 7.6-7.8 and and added with surfactant to imrove spreading and preservative to inhibit microbial growth.
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