Question

which of these is a component used in our PCR reaction? A. wash buffer B. DNA...

which of these is a component used in our PCR reaction?

A. wash buffer

B. DNA polymerase

C. elution Buffer

D. all options are correct

Homework Answers

Answer #1

Ans :- all options are correct

For PCR reaction the components are used that are given below -

PCR PRIMERS

TEMPLATE DNA

PCR BUFFER

TAQ POLYMERASE

NUCLEOTIDES

Temperature is necessary to separate two complementary strands of DNA and Dna polymerase is used as a component in PCR reaction that can tolerate high temperature upto 95 degree C.Wash buffer is a tris buffer solution Ph is near to 7.6-7.8 and and added with surfactant to imrove spreading and preservative to inhibit microbial growth.

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