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Describe the steps of the chromatin immunoprecipitation assay.

Describe the steps of the chromatin immunoprecipitation assay.
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Answer #1

Chromatin immunoprecipitation assay is a technique for investigation whether specific proteins are associated with specific genomic regions. It is actually done to determine if any interactions are present between DNA and protein in a cell.

Steps are:-

Crosslinking of DNA to histone protein in the living cell and cell lysis to release chromatin.

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Fragmentation of chromatin with nuclease digestion and sonication.

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Enrichment of target chromatin using an antibody specific protein (Antibody- magnetic bead complex)

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Purification of associated DNA by removal of the protein with protease and elution of DNA.

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Analysis of DNA by polymerase chain reaction (PCR).

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