Question

(a) Does the signal for any of the protein samples fall over the top of the standard curve. What could cause this? If there were, what might you do?

(b) Are the signals for the samples close to the background signal (zero protein value on standard curve)? What could cause this? If there were, what might you do?

Answer 1

When the absorbtion is huge that means much more protein is present in small volume which means higher concentration of protein ,then signal can fall over the top of the stranded curve.

If this happens then you have to dilute the sample.

B)if the signals is close to the background noise then it mRNAs the protein sample has very low concentrations of protein and thus the absorbtion is Very less .

It can be overcome by increasing the voltage of instruments so that it can measure very low absorbtion or the sample concentration will have to increase like if in a step protein is needed less you have to give more protein.