Question

You are given the coding strand sequence of the region that you are interested in PCR...

You are given the coding strand sequence of the region that you are interested in PCR amplifying. Design forward and reverse primers using the sequences in bold. Your primer sequences should be written in a 5' → 3' direction.

5' - TACGTA GTCGAGATCTTACGATAAC CGTATAG ... CGTAGGTA CATGGTTCATGTCGAAGTC TAG -3'

the ... contains the region of interest

Science   Biology   
0 0
Add a commentTranscribed image text
Next >

Homework Answers

Answer #1

0 0
Know the answer?
Your Answer:

Post as a guest

Your Name:

What's your source?

Earn Coins

Coins can be redeemed for fabulous gifts.

Log In

Sign Up

Not the answer you're looking for?
Ask your own homework help question
Similar Questions
PCR Questions What gives DNA strands its polarity? To answer, tell me what the “ 5’...
PCR Questions What gives DNA strands its polarity? To answer, tell me what the “ 5’ ” and “ 3’ ” ends are referring to in chemical terms.   Diagram a “template” and the primers for a PCR reaction, including the 5’ and 3’ notations as appropriate. Will the “forward” primer have the same sequence as the coding strand or complement strand? Will the “reverse” primer have the same sequence as the coding strand or complement strand? After running PCR, a...
You are performing a simple polymerase chain reaction (PCR) to amplify ANY 300 - 500 bp...
You are performing a simple polymerase chain reaction (PCR) to amplify ANY 300 - 500 bp region (for example nucleotide 5 to 555 or 20 to 575). Using your primers, you will run a theoretical PCR reaction, and then run the product on an agarose gel to determine if your PCR reaction worked correctly. CAGGAAACAGCTATGACCATGATTACGCCAAGCTTGGTACCGAGCTCGGATCCACTAGTA ACGGCCGCCAGTGTGCTGGAATTCGGCTTCCCGGAGCCGGACCGGGGCCACCGCGCCCGC TCTGCTCCGACACCGCGCCCCCTGGACAGCCGCCCTCTCCTCCAGGCCCGTGGGGCTGGC CCTGCACCGCCGAGCTTCCCGGGATGAGGGCCCCCGGTGTGGTCACCCGGCGCGCCCCAG GTCGCTGAGGGACCCCGGCCAGGCGCGGAGATGGGGGTGCACGAATGTCCTGCCTGGCTG TGGCTTCTCCTGTCCCTGCTGTCGCTCCCTCTGGGCCTCCCAGTCCTGGGCGCCCCACCA CGCCTCATCTGTGACAGCCGAGTCCTGGAGAGGTACCTCTTGGAGGCCAAGGAGGCCGAG AATATCACGACGGGCTGTGCTGAACACTGCAGCTTGAATGAGAATATCACTGTCCCAGAC ACCAAAGTTAATTTCTATGCCTGGAAGAGGATGGAGGTCGGGCAGCAGGCCGTAGAAGTC TGGCAGGGCCTGGCCCTGCTGTCGGAAGCTGTCCTGCGGGGCCAGGCCCTGTTGGTCAAC TCTTCCCAGCCGTGGGAGCCCCTGCAGCTGCATGTGGATAAAGCCGTCAGTGGCCTTCGC AGCCTCACCACTCTGCTTCGGGCTCTGCGAGCCCAGAAGGAAGCCATCTCCCCTCCAGAT CAGCCTGTCCCATGGACACTCCAGTGCCAGCAATGACATCTCAGGGGCCAGAGGAACTGT CCAGAGAGCAACTCTGAGATCTAAGGATGTCACAGGGCCAACTTGAGGGCCCAGAGCAGG AAGCATTCAGAGAGCAGCTTTAAACTCAGGGACAGAGCCATGCTGGGAAGACGCCTGAGC TCACTCGGCACCCTGCAAAATTTGATGCCAGGACACGCTTTGGAGGCGATTTACCTGTTT TCGCACCTACCATCAGGGACAGGATGACCTGGAGAACTTAGGTGGCAAGCTGTGACTTCT CCAGGTCTCACGGGCATGGGCACTCCCTTGGTGGCAAGAGCCCCCTTGACACCGGGGTGG TGGGAACCATGAAGACAGGATGGGGGCTGGCCTCTGGCTCTCATGGGGTCCAAGTTTTGT GTATTCTTCAACCTCATTGACAAGAACTGAAACCACCAAAAAAAAAAAAAAAAGCCGAAT TCTGCAGATATCCATCACACTGGCGGCCGCTCGAGCATGCATCTAGAGGGCCCAATTCGC CCTATAGTGAGTCGTATTACAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAA TCTTCCCAGCCGTGGGAGCCCCTGCAGCTGCATGTGGATAAAGCCGTCAGTGGCCTTCGC AGCCTCACCACTCTGCTTCGGGCTCTGCGAGCCCAGAAGGAAGCCATCTCCCCTCCAGAT GCGGCCTCAGC Questions...
The forward and reverse primers for PCR amplification have the following sequences: Forward: 5'-CTATGAGTTACTCACATG-3', Reverse: 5'-...
The forward and reverse primers for PCR amplification have the following sequences: Forward: 5'-CTATGAGTTACTCACATG-3', Reverse: 5'- GAAAGCATCCAGGCCCGG-3'. Based on the manually calculated melting temperatures, would you recommend using these primers together? Why or why not?
1. Suppose you use the forward primer 5' AATGAAA 3' and the reverse primer 5' ATCAGGG...
1. Suppose you use the forward primer 5' AATGAAA 3' and the reverse primer 5' ATCAGGG 3' to amplify the double-stranded DNA fragment 5' ATGAAATTTGGGCCCTGA 3'. What will be the sequence of the most abundant amplicon in the PCR after 40 cycles? 2. Suppose you use the forward primer 5' TGAAA 3' and the reverse primer 5' CAGGG 3' to amplify the double-stranded DNA fragment 5' ATGAAATTTGGGCCCTGA 3'. What will be the sequence of the most abundant amplicon in the...
Here is the nucleotide sequence of one strand of a fragment of double-stranded DNA, which will...
Here is the nucleotide sequence of one strand of a fragment of double-stranded DNA, which will be amplified by PCR using short primers:      5' TGGCAGTCGATGCTGCTAGCTGGCCTTGAGTCGTGC 3'      Primers that could be used to amplify this entire DNA fragment are: A) 5’ GACTGCCA 3’ & 5’ GCACGACT 3’ B) 5’ GACTGCCA 3’ & 5’ AGTCGTGC 3’ C) 5’ TGGCAGTC 3’ & 5’ GCACGACT 3’ D) 5’ TGGCAGTC 3’ & 5’ AGTCGTGC 3’ E) 5’ TGGCAGTC 3’ & 5’ CGTGCTGA 3’
5’…TAC ACC GAT GGA TAA GTC…3’ DNA Coding strand 3’…ATG TGG CTA CCT ATT CAG…5’ DNA...
5’…TAC ACC GAT GGA TAA GTC…3’ DNA Coding strand 3’…ATG TGG CTA CCT ATT CAG…5’ DNA template strand Use the information above to answer the following questions. Write the sequences of the two double-stranded daughter DNAs which will be replicated from the sequence above. Indicate which sequences are directly from the parent. Show accurate direction of each sequence. 0.5 points Write the sequence of the mRNA strand that will be transcribed from the above DNA sequence. Show accurate direction of...
5’…TAC ACC GAT GGA TAA GTC…3’ DNA Coding strand 3’…ATG TGG CTA CCT ATT CAG…5’ DNA...
5’…TAC ACC GAT GGA TAA GTC…3’ DNA Coding strand 3’…ATG TGG CTA CCT ATT CAG…5’ DNA template strand Use the information above to answer the following questions. Write the sequences of the two double-stranded daughter DNAs which will be replicated from the sequence above. Indicate which sequences are directly from the parent. Show accurate direction of each sequence. 0.5 points Write the sequence of the mRNA strand that will be transcribed from the above DNA sequence. Show accurate direction of...
5’…TAC ACC GAT GGA TAA GTC…3’ DNA Coding strand 3’…ATG TGG CTA CCT ATT CAG…5’ DNA...
5’…TAC ACC GAT GGA TAA GTC…3’ DNA Coding strand 3’…ATG TGG CTA CCT ATT CAG…5’ DNA template strand Use the information above to answer the following questions. Write the sequences of the two double-stranded daughter DNAs which will be replicated from the sequence above. Indicate which sequences are directly from the parent. Show accurate direction of each sequence. 0.5 points Write the sequence of the mRNA strand that will be transcribed from the above DNA sequence. Show accurate direction of...
A Drosophila gene produces an mRNA that begins with the following base sequence, 5’CAGUCUCCGACGU..... and ends...
A Drosophila gene produces an mRNA that begins with the following base sequence, 5’CAGUCUCCGACGU..... and ends in a poly-A tail. A full-length cDNA copy of this mRNA can be made and amplified by using one primer and the appropriate enzyme to make a first strand cDNA, then adding a second primer and another enzyme to make and amplify double-stranded DNA in a polymerase chain reaction. Which primers and enzymes should be used first and second? First primer First enzyme Second...
You will need to read the following article “In Iceland’s DNA Clues to Disease” (in your...
You will need to read the following article “In Iceland’s DNA Clues to Disease” (in your problem sets folder in CANVAS). The article describes the results obtained by researchers at deCODE, a genetics firm in Iceland. Their goal was to harness Iceland’s unique homogenous population with low genetic diversity coupled with excellent geneology records to uncover rare genetic mutations that could lead to disease. The research article (known as a primary source) for the information above was published in the...
ADVERTISEMENT
Need Online Homework Help?

Get Answers For Free
Most questions answered within 1 hours.

Ask a Question
ADVERTISEMENT