Question

Microbiology Unknown Bacteria Sample: you received a mixed broth culture and were instructed to identify all organisms present in that mixture. What are the first steps you should do to accomplish your goal?

Answer 1

If u get an unkown mixed broth of culture, the first few steps done are as follows:-

1. Isolation- it is the first step done to isolate pure bacterial cultures. It can be done by various methods like streaking,dilution, plating etc.

2. Staining reaction-It is the basic technique used to identify microorganisms by using Methylene blue.

Gram staining os a differential staining technique to differentiate the bacterium whether it is gram positive or gram negetive.

3. Biochemical reactions: they are used to differentiate the members of enterobactericae. They are done by IMViC tests like indole test, methyl red test,Voges proskauer test and citrate test.

Hope this helps you. Thank you!?