Question

Identifying an unknown bacteria

step 1: there are 2 isolates on the plate - 1 small grey and 1 yellow/gold pinpoint

What should you do next to identify the bacteria?

Answer 1

Step 2 :-

Note for the colony characteristics from the given plate for each of the bacteria.

Step 3 :-

Gram staining to study the morphological characteristics of the bacteria (or any special stain can also be performed).

Step 4 :-

Perform Motility test.

Step 5 :-

Perform Biochemical Tests (Such as catalase, coagulase, oxidase, Glucose fermentation test, TSI, DNAse, Phosphatase, Mannitol sugar fermentation, gelatin liquefaction etc.)

Step 6 :-

Test for enzymes and toxin production.

Step 7 :-

Anti microbial susceptibility testing.

Step 8:-

Antigen detection can be done at last.