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Why are the BLASTP results not in exactly the same order as the BLASTN results, even...

Why are the BLASTP results not in exactly the same order as the BLASTN results, even though you were using the same gene for both searches?

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Answer #1

BLAST is a Basic Local Alignment Search Tool which allows the user to compare or find similarity between two biological sequences. BLASTP is a program which returns most similar protein sequences to a specific protein sequence query. While BLASTN is a program which returns most similar nucleotide sequences to a specific sequence query from the nucleotide database.

The sole reason why the same gene results differs in BLASTP and BLASTN is because of the way they are presented.

In BLASTP the gene sequence is expressed in its amino acid form i.e. it is written in amino acid codes (A,B,C,D,E,F,G,H,I,J,K,L,M,N,O,P,Q,R,S,T,U,V,W,X,Y,Z)

While in BLASTN the gene sequence is expressed by their 4 bases - Adenine (A), Guanine (G), Cytosine (C), Thymine ()T, and Uracil (U) (in case of RNA sequence)

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