The events of the electron transport chain involve NADH and FADH, which act as electron transporters as they flow through the inner membrane space. In complex I, electrons are passed from NADH to the electron transport chain, where they flow through the remaining complexes. NADH and FADH2 are he oxidized carriers in this process.
As the amount of the hydronium ion in the inter membrane matrix increases the pH of the inter membrane matrix decreases.
Energy obtained through the transfer of electrons down the electron transport chain is used to pump protons from the mitochondrial matrix into the intermembrane space, creating an electrochemical proton gradient (ΔpH) across the inner mitochondrial membrane
So,option 4 ( all of the above ) is the correct answer.
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