Question

Serotonin (5-HT) causes a dose-dependent contraction of some blood vessels that cause migraine. Suppose the equilibrium constant, Ka, were 3µM, using the classic simple receptor theory equation calculate what the % max response would be seen at a concentration of 1µM serotonin.

Answer 1

Answer:

• Equilibrium constant Ka for Serotonin = 3uM
• Concentration of Serotonin = 1uM

Relationship between the concentration of the agonist and the response that it elicits is:

• Where, Ea = Response
• Em = Maximum Response
• [A] = Agonist concentration
• Ka = dissociation constant

Therefore, % maximal response that would be seen at a concentration of 1uM Serotonin is 25%