Question

The first test performed on bacteria is a gram stain. The stain is then later used...

The first test performed on bacteria is a gram stain. The stain is then later used to classify the bacteria based on the differences in the cell wall composition. Explain how a gram stain is performed.

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Answer #1

Gram staining is a differential staining method.We can differentiate bacteria on the basis of the cell wall with the help of Gram staining method.Gram negative bacteria appear pink and gram positive bacteria appear violet under microscope (due to presence of peptidoglycan layer which, retains crystal violet).

The procedure is shown in in the attached picture in a flow chart.

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