3. When the base composition of double-stranded DNA from Micrococcus luteus was determined, 37.5% of the bases were found to be cytosine. What is the percentage of adenine in the DNA of this organism? _____ % adenine
4. A region along a DNA strand that is transcribed contains no A. What base will be missing in the corresponding region of the mRNA? _____________
Answer=
3. When the base composition of double-stranded DNA from Micrococcus luteus was determined,
37.5% of the bases were found to be cytosine.
C=37.5%
according to chargaffs law DNA from any organism should have 1:1 ratio of pyrimidine & purine.
Guanine should be equal to Cytosine & Adenine should be equal to Thymine.
Guanine = 37.5%
Total bases present in DNA are
Adenine + Cytosine + Guanine + Thymine = 100%
But A=T
A +A +37.5 +37.5 =100
2Adenine = 100- 75
2Adenine = 25
Adenine =25/2 = 12.5 %
should contain 12.5 % of Adenine.
4. A region along a DNA strand that is transcribed contains no A. What base will be missing in the corresponding region of the mRNA? _____________
answer= Uracil
because Adenine in DNA is transcribed into Uracil in case of RNA
adenine pairs with Uracil in RNA.
if Adenine is absent in DNA ,Uracil is missing from RNA.
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