Recall that you used agarose powder, 1X TBE buffer, and a fluorescent dye, GreenGlo, to make an agarose gel in lab. Calculate the amounts for following reagents to make a 2.0% - 50 ml agarose gel. Answer the amounts for GreenGlo in microliters and the amounts for TBE buffer and diluent in milliliters. (3 points)
a. Agarose powder (g) (1 point)
b. 20,000X GreenGlo (μl) (1 point)
c. 10X TBE Buffer. Include the amount of diluent. (ml) (1 point)
Get Answers For Free
Most questions answered within 1 hours.