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If you would like to load 10 µL of your sample onto a SDS-PAGE gel, how...

If you would like to load 10 µL of your sample onto a SDS-PAGE gel, how much 6X Laemmli dye would you need to add to your sample?

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Answer #1

The final concentration of the dye in the loading sample is 1X,Now,Final volume is 10l

If 6X is the stock concentration of the dye dilute as much of dye that the final concentration in the sample is 1X

Therefore according to the dilution law

i.e. M1 X V1 = M2 X V2

6 X V1 = 1 X 10

=1.66 = ~1.7

So if you load the sample of 10l then 1.7l is dye and 8.3l is your sample.

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